restriction digest meaning in English
限制酶切消化
Examples
- Cloning the gene into a pgex expression vector clone the hbrp gene using pcr , restriction digest the pgex - 5x - 1 vectors and pcr product with bamh i and xho i , recovery the pcr product and pgex - 5x - 1 vectors
纯化样品样品上柱， pbs洗柱后，用洗脱buf企r将蛋白洗脱下来。利用蛋白收集器，收集蛋白，浓缩结果1 - The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest . then the recombinant plasmid was identified by endonuclease analysis , pcr ampliation and dna sequencing . the report showed that the recombinant plasmid had right open reading frame
重组质粒经酶切鉴定， pcr鉴定和测序，结果证实猪肺炎支原体黏附因子p97基因的抗原决定簇r1区定向插入了质粒pet - 32a ( + ) ，且阅读框架正确。